Analysis
qPCR Molecular Analysis
Real-time PCR for gene expression analysis, nucleic acid quantification and target sequence detection using SYBR Green and probe-based chemistries.
In short
Real-time PCR for gene expression analysis, nucleic acid quantification and target sequence detection using SYBR Green and probe-based chemistries. The qPCR molecular analysis service is run on a Bio-Rad C1000 Touch thermal cycler with the CFX96 optical module. Melt curve analysis confirms product specificity, and multichannel optics allow multiplexing of several targets in one well.
The qPCR molecular analysis service is run on a Bio-Rad C1000 Touch thermal cycler with the CFX96 optical module. Amplification of target DNA or cDNA sequences is monitored in real time using SYBR Green or TaqMan probe chemistries, and quantification cycle (Cq) values are converted into absolute quantities via standard curves or relative expression via the ΔΔCq method. Melt curve analysis confirms product specificity, and multichannel optics allow multiplexing of several targets in one well. Typical questions include changes in cellular gene expression after exposure to biomaterials or nanoparticles, microbial load and species detection, GMO and pathogen screening, plasmid copy number and nucleic acid quality. It addresses biotechnology, pharmaceutical, food, agricultural and environmental organisations and academic groups in nanobiotechnology and the life sciences.
What you receive
Amplification curves, Cq values and melt curve plots
Standard curve parameters (slope, efficiency, R²) or ΔΔCq relative expression tables
Negative control, no-template control and reference gene evaluation
Method summary covering primers/probes, reaction mix and cycling conditions
Raw data (CFX export, Excel) and PDF report
How it works
Request and scoping
Target genes, sample type, chemistry (SYBR/probe), reference genes and the statistical design (replicates, controls) are planned with you.
Sample reception and preparation
Samples are received with cold-chain records; nucleic acid quality and concentration are checked, cDNA is synthesised where needed and the plate layout is prepared.
Measurement
Reactions are set up in technical replicates with appropriate controls; qPCR cycling and melt curve analysis are run on the CFX96 system.
Reporting
Cq data are processed in line with the MIQE guidelines; quantity or expression results are presented in the standard analysis report.
Pricing
€160
per sample
| 1+ | €160 |
| 5+ | €144 |
| 10+ | €128 |
Lead time 3–7 business days
Final pricing depends on sample count, preparation and reporting scope. You receive an itemised proposal.
Prices are a guide and may change with any additional requests. Our team will contact you to confirm the scope and the final price.Request this service
Describe your sample and the decision it supports. We reply with a technical proposal.
Request analysisInstruments used
- qPCR
- Bio-Rad C1000 Touch + CFX96 optics
Questions
Frequently asked questions
How do I send samples for qPCR Molecular Analysis?
Fill in the analysis request form. Our team replies with preparation and shipping instructions for your sample type, and we confirm together that the sample is suitable before you send it.
How long does qPCR Molecular Analysis take?
It depends on the number of samples and laboratory workload. The estimated time on this page is a reference; once we review your request we share a firm schedule.
How is the price set?
The price on this page is a list price. It may change with the number of samples and any additional requests; after we agree the scope, the final price is shared in a proposal.
How do I receive the results?
Results are delivered as a written report. You can follow the status of your request in your account and through email notifications.
Can I request this from abroad?
Yes. We accept requests in Turkish, English, Arabic and Persian, and reply in the language you prefer.
Can I see your quality documents?
We develop our quality processes with reference to ISO/IEC 17025. For the current documents and scope details, contact us and we will send them in writing.
Instruments used
Life science
Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module
Real-time quantitative PCR in a 96-well format with five-colour multiplex fluorescence detection for gene expression, pathogen detection and genotyping.
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Consulting
Method Development and Validation Consulting
Consulting for designing, optimising, validating and documenting analytical test methods specific to your product and process in line with ISO/IEC 17025.
Further reading
Characterization
qPCR You Can Trust: Samples, Controls and Shipping
A trustworthy qPCR result is decided long before anything is pipetted into a plate. A practical, MIQE-aligned guide to sample integrity, controls, replicates and what to send to the laboratory.
November 9, 20267 min read
Request this service
Describe your sample and the decision it supports. We reply with a technical proposal.