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Life science

Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module

Real-time quantitative PCR in a 96-well format with five-colour multiplex fluorescence detection for gene expression, pathogen detection and genotyping.

96-well real-time PCR instrument in a molecular biology laboratory
Photo: İnformatic via Wikimedia Commons, CC BY-SA 3.0
Operational
ISO 20395

In short

Real-time quantitative PCR in a 96-well format with five-colour multiplex fluorescence detection for gene expression, pathogen detection and genotyping. The Peltier-based block provides fast heating and cooling ramps and a temperature gradient so that primer annealing can be optimised in a single run.

Block format
96-well, 0.2 mL
Optical detection
6 filtered LED/photodiode pairs, up to 5-target multiplex
Reaction volume
10 – 50 µL

Services on this instrument

Prices are a guide and may change with any additional requests. Our team will contact you to confirm the scope and the final price.

Overview

The Bio-Rad C1000 Touch is a touch-screen thermal cycler base with gradient capability; fitted with the CFX96 optical reaction module it becomes a real-time PCR system that scans every well of the 96-well block independently with six filtered LED/photodiode pairs for multiplex detection of up to five targets. The Peltier-based block provides fast heating and cooling ramps and a temperature gradient so that primer annealing can be optimised in a single run. SYBR Green and probe-based chemistries support absolute and relative quantification, melt-curve analysis, allelic discrimination and end-point detection; the software reports standard curves, efficiency calculations and ΔΔCq-based gene expression analysis. Applications include transcript-level assessment of nanomaterial effects on cells, pathogen-load determination on antimicrobial surfaces, GMO and species verification, and molecular validation in biomaterials research.

Measurement principle

Capabilities

  • Absolute and relative quantification with SYBR Green and probe-based chemistries

  • Multiplex fluorescence detection of up to five targets

  • Melt-curve analysis and allelic discrimination (SNP genotyping)

  • Single-run primer optimisation using the temperature gradient

  • ΔΔCq gene expression analysis and standard-curve reporting

  • End-point PCR and conventional thermal cycling protocols

Suitable samples

  • cDNA from cell cultures and tissues

  • Bacterial, fungal and viral nucleic acid extracts

  • DNA from food, feed and agricultural products

  • Environmental and surface swab samples

  • Plasmid and synthetic DNA standards

Technical specifications

Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module — Technical specifications
Block format96-well, 0.2 mL
Optical detection6 filtered LED/photodiode pairs, up to 5-target multiplex
Reaction volume10 – 50 µL
Temperature range0 – 100 °C
Maximum ramp rate5 °C/s
Gradient range1 – 24 °C
SoftwareCFX Maestro for quantification, melt-curve and gene expression analysis

Services on this instrument

Prices are a guide and may change with any additional requests. Our team will contact you to confirm the scope and the final price.
Manufacturer
Bio-Rad
Model
C1000 Touch + CFX96 optics
Catalogue no.
31
Reference standards
ISO 20395

Quick answers

qPCR: key facts

What is Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module used for?

It is used for: Absolute and relative quantification with SYBR Green and probe-based chemistries, Multiplex fluorescence detection of up to five targets, Melt-curve analysis and allelic discrimination (SNP genotyping), Single-run primer optimisation using the temperature gradient, ΔΔCq gene expression analysis and standard-curve reporting, and End-point PCR and conventional thermal cycling protocols.

Which samples can Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module analyse?

Suitable samples: cDNA from cell cultures and tissues, Bacterial, fungal and viral nucleic acid extracts, DNA from food, feed and agricultural products, Environmental and surface swab samples, and Plasmid and synthetic DNA standards.

Which standards does Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module relate to?

Relevant standards: ISO 20395.

Which NAKTEM services use Bio-Rad C1000 Touch Thermal Cycler with CFX96 Real-Time PCR (qPCR) Optical Module?

qPCR Molecular Analysis and Instrument Operator Training.

What results do I receive?

Typical deliverables: Amplification curves, Cq values and melt curve plots, Standard curve parameters (slope, efficiency, R²) or ΔΔCq relative expression tables, Negative control, no-template control and reference gene evaluation, Method summary covering primers/probes, reaction mix and cycling conditions, and Raw data (CFX export, Excel) and PDF report.

  • Characterization

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    November 9, 20267 min read

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