Sample preparation and processing
Ultracentrifugation and Separation
High-speed ultracentrifugation to separate, purify and concentrate nanoparticles, exosomes, viruses, proteins and polymer fractions from suspensions.
In short
High-speed ultracentrifugation to separate, purify and concentrate nanoparticles, exosomes, viruses, proteins and polymer fractions from suspensions. The ultracentrifugation and separation service uses a Beckman Coulter Optima XPN-100 ultracentrifuge at speeds up to 100,000 rpm under temperature-controlled conditions. Rotor, g-force, run time and temperature are calculated from the target particle size, density and medium viscosity.
The ultracentrifugation and separation service uses a Beckman Coulter Optima XPN-100 ultracentrifuge at speeds up to 100,000 rpm under temperature-controlled conditions. Differential pelleting separates nanoparticles and exosomes from their medium, sucrose or iodixanol density gradients fractionate by size or density, and isopycnic centrifugation provides high-purity isolation. Rotor, g-force, run time and temperature are calculated from the target particle size, density and medium viscosity. Typical needs include removing free ligands and solvent from synthesised nanoparticles, separating size fractions, purifying exosomes and viral vectors, and concentrating protein complexes and polymer dispersions. It serves nanomaterial synthesis, biotechnology, pharmaceutical, vaccine and academic life science groups; fractions can be verified by particle size and qPCR analysis.
What you receive
Separated and labelled fractions (pellet, supernatant or gradient bands)
Process record covering rotor, speed (rpm/g), run time, temperature and gradient composition
Volume and mass balance with recovery notes
Post-separation particle size or concentration verification on request
Process summary report (PDF)
How it works
Request and scoping
Sample type, target fraction, volume, biosafety level and purity expectation are agreed; a protocol and rotor selection are proposed.
Sample reception and preparation
Samples are received under cold chain; clarifying pre-spins are performed, gradients prepared and tubes precisely balanced.
Measurement
Centrifugation is run at the defined speed, duration and temperature; run parameters are logged and fractions are collected in a controlled manner.
Reporting
Fractions are labelled and delivered with the process record; recovery and purity findings are discussed with you on request.
Pricing
from
€700
per batch
Lead time 7–15 business days
Final pricing depends on sample count, preparation and reporting scope. You receive an itemised proposal.
Prices are a guide and may change with any additional requests. Our team will contact you to confirm the scope and the final price.Request this service
Describe your sample and the decision it supports. We reply with a technical proposal.
Request analysisInstruments used
- Ultracentrifuge
- Beckman Coulter Optima XPN-100
Questions
Frequently asked questions
How do I send samples for Ultracentrifugation and Separation?
Fill in the analysis request form. Our team replies with preparation and shipping instructions for your sample type, and we confirm together that the sample is suitable before you send it.
How long does Ultracentrifugation and Separation take?
It depends on the number of samples and laboratory workload. The estimated time on this page is a reference; once we review your request we share a firm schedule.
How is the price set?
The price on this page is a list price. It may change with the number of samples and any additional requests; after we agree the scope, the final price is shared in a proposal.
How do I receive the results?
Results are delivered as a written report. You can follow the status of your request in your account and through email notifications.
Can I request this from abroad?
Yes. We accept requests in Turkish, English, Arabic and Persian, and reply in the language you prefer.
Can I see your quality documents?
We develop our quality processes with reference to ISO/IEC 17025. For the current documents and scope details, contact us and we will send them in writing.
Instruments used
Separation and purification
Beckman Coulter Optima XPN-100 Preparative Ultracentrifuge
Ultracentrifugation up to 100,000 rpm and 802,000 × g for separating, purifying and density-gradient fractionating nanoparticles, exosomes, viruses and macromolecules.
Related services
Sample preparation and processing
Cross-Flow Filtration and Concentration
Tangential flow filtration (TFF) for gentle concentration, diafiltration and buffer exchange of nanoparticle, protein and polymer dispersions at laboratory scale.
Analysis
Particle Size Distribution Analysis
Laser diffraction measurement of particle size distribution in powders, suspensions and emulsions from the nanometre to the millimetre range.
Analysis
qPCR Molecular Analysis
Real-time PCR for gene expression analysis, nucleic acid quantification and target sequence detection using SYBR Green and probe-based chemistries.
Request this service
Describe your sample and the decision it supports. We reply with a technical proposal.