Separation and purification
CRP-02A Cross-Flow (Tangential Flow) Membrane Filtration System
Fouling-resistant concentration, diafiltration and size-based fractionation of nanoparticle, protein and colloidal suspensions through MF and UF membranes.
In short
Fouling-resistant concentration, diafiltration and size-based fractionation of nanoparticle, protein and colloidal suspensions through MF and UF membranes. The CRP-02A operates on the cross-flow (tangential flow) filtration principle, in which the feed is pumped tangentially across the membrane surface so that retained species are continuously swept away, largely preventing the cake formation and fouling associated with dead-end filtration.
- Filtration principle
- Cross-flow / tangential flow (TFF)
- Membrane formats
- Hollow fibre and flat-sheet cassette
- Separation range
- Microfiltration to ultrafiltration (selectable MWCO)
Services on this instrument
Cross-Flow Filtration and Concentration
from €8307–15 business daysRequest this serviceService details
Overview
The CRP-02A operates on the cross-flow (tangential flow) filtration principle, in which the feed is pumped tangentially across the membrane surface so that retained species are continuously swept away, largely preventing the cake formation and fouling associated with dead-end filtration. The system accepts hollow-fibre or flat-sheet cassette modules with pore sizes and molecular weight cut-offs (MWCO) spanning microfiltration to ultrafiltration. In concentration mode, permeate is removed to reduce product volume; in diafiltration mode, buffer exchange, desalting or wash-out of residual reagents is performed at constant volume. Transmembrane pressure, cross-flow rate and permeate flux are monitored and reported. Typical applications include purification of nanoparticle and quantum dot suspensions, washing of graphene oxide and CNT dispersions, concentration of proteins and polysaccharides, and enrichment of extracellular vesicles.
Measurement principle
Capabilities
Concentration of suspensions through micro- and ultrafiltration membranes
Buffer exchange and desalting by constant-volume diafiltration
Washing and purification of nanoparticle and graphene oxide dispersions
Size-based fractionation by selection of membrane MWCO
Monitoring of transmembrane pressure, flow rate and permeate flux
Compatibility with hollow-fibre and flat-sheet cassette modules
Suitable samples
Nanoparticle and quantum dot suspensions
Graphene oxide and CNT dispersions
Protein and enzyme solutions
Polysaccharide and biopolymer solutions
Extracellular vesicle-containing media
Fermentation and cell culture broths
Technical specifications
| Filtration principle | Cross-flow / tangential flow (TFF) |
|---|---|
| Membrane formats | Hollow fibre and flat-sheet cassette |
| Separation range | Microfiltration to ultrafiltration (selectable MWCO) |
| Operating modes | Concentration, diafiltration, clarification |
| Monitored parameters | Transmembrane pressure, cross-flow rate, permeate flux |
| Process scale | Laboratory and pilot-scale batch processing |
Services on this instrument
Cross-Flow Filtration and Concentration
from €8307–15 business daysRequest this serviceService details
- Model
- CRP-02A
- Catalogue no.
- 6
Quick answers
Cross-Flow Filtration: key facts
What is CRP-02A Cross-Flow (Tangential Flow) Membrane Filtration System used for?
It is used for: Concentration of suspensions through micro- and ultrafiltration membranes, Buffer exchange and desalting by constant-volume diafiltration, Washing and purification of nanoparticle and graphene oxide dispersions, Size-based fractionation by selection of membrane MWCO, Monitoring of transmembrane pressure, flow rate and permeate flux, and Compatibility with hollow-fibre and flat-sheet cassette modules.
Which samples can CRP-02A Cross-Flow (Tangential Flow) Membrane Filtration System analyse?
Suitable samples: Nanoparticle and quantum dot suspensions, Graphene oxide and CNT dispersions, Protein and enzyme solutions, Polysaccharide and biopolymer solutions, Extracellular vesicle-containing media, and Fermentation and cell culture broths.
Which NAKTEM services use CRP-02A Cross-Flow (Tangential Flow) Membrane Filtration System?
Cross-Flow Filtration and Concentration.
What results do I receive?
Typical deliverables: Concentrated or diafiltered product (retentate) and permeate sample on request, Process record covering membrane type/MWCO, transmembrane pressure, cross-flow rate and flux–time log, Volume concentration factor, diafiltration volumes and recovery calculation, Product concentration or particle size verification on request, and Process summary report (PDF).
Related services
Sample preparation and processing
Cross-Flow Filtration and Concentration
Tangential flow filtration (TFF) for gentle concentration, diafiltration and buffer exchange of nanoparticle, protein and polymer dispersions at laboratory scale.
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