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Separation and purification

Beckman Coulter Optima XPN-100 Preparative Ultracentrifuge

Ultracentrifugation up to 100,000 rpm and 802,000 × g for separating, purifying and density-gradient fractionating nanoparticles, exosomes, viruses and macromolecules.

Floor-standing preparative ultracentrifuge in a laboratory
Photo: Karbohut via Wikimedia Commons, CC BY-SA 4.0
Operational

In short

Ultracentrifugation up to 100,000 rpm and 802,000 × g for separating, purifying and density-gradient fractionating nanoparticles, exosomes, viruses and macromolecules. The Beckman Coulter Optima XPN-100 is a floor-standing preparative ultracentrifuge whose vacuum-enclosed, frictionless induction-drive rotor system reaches 100,000 rpm and relative centrifugal forces of up to 802,000 × g.

Maximum speed
100,000 rpm
Maximum RCF
802,000 × g
Temperature range
0 – 40 °C

Services on this instrument

Prices are a guide and may change with any additional requests. Our team will contact you to confirm the scope and the final price.

Overview

The Beckman Coulter Optima XPN-100 is a floor-standing preparative ultracentrifuge whose vacuum-enclosed, frictionless induction-drive rotor system reaches 100,000 rpm and relative centrifugal forces of up to 802,000 × g. Fixed-angle, swinging-bucket and near-vertical rotors support both rate-zonal and isopycnic density-gradient separations, in which particles are fractionated by size and buoyant density in sucrose, iodixanol or CsCl gradients. Temperature is precisely controlled between 0 and 40 °C to protect heat-sensitive biological material. Typical applications include pelleting of metal oxide and polymer nanoparticles, size classification of carbon nanotube and graphene dispersions, isolation of exosomes and extracellular vesicles, purification of viruses and viral vectors, and preparation of liposomes and protein complexes. Programmable acceleration and deceleration profiles preserve gradient integrity, and every run is logged.

Measurement principle

Capabilities

  • High-speed sedimentation up to 100,000 rpm and 802,000 × g

  • Isopycnic and rate-zonal separation in sucrose, iodixanol and CsCl gradients

  • Size classification of nanoparticle, CNT and graphene dispersions

  • Purification of exosomes, extracellular vesicles and viruses

  • Processing of heat-sensitive samples under 0–40 °C temperature control

  • Programmable acceleration / deceleration profiles with run logging

Suitable samples

  • Nanoparticle suspensions

  • Carbon nanotube and graphene dispersions

  • Exosomes and extracellular vesicles

  • Viruses and viral vectors

  • Liposomes and lipid nanoparticles

  • Protein, nucleic acid and macromolecule solutions

Technical specifications

Beckman Coulter Optima XPN-100 Preparative Ultracentrifuge — Technical specifications
Maximum speed100,000 rpm
Maximum RCF802,000 × g
Temperature range0 – 40 °C
DriveFrictionless induction drive under vacuum
Rotor typesFixed-angle, swinging-bucket, near-vertical
Programme controlTouch-screen interface, acceleration/deceleration profiles, run log

Services on this instrument

Prices are a guide and may change with any additional requests. Our team will contact you to confirm the scope and the final price.
Manufacturer
Beckman Coulter
Model
Optima XPN-100
Catalogue no.
4

Quick answers

Ultracentrifuge: key facts

What is Beckman Coulter Optima XPN-100 Preparative Ultracentrifuge used for?

It is used for: High-speed sedimentation up to 100,000 rpm and 802,000 × g, Isopycnic and rate-zonal separation in sucrose, iodixanol and CsCl gradients, Size classification of nanoparticle, CNT and graphene dispersions, Purification of exosomes, extracellular vesicles and viruses, Processing of heat-sensitive samples under 0–40 °C temperature control, and Programmable acceleration / deceleration profiles with run logging.

Which samples can Beckman Coulter Optima XPN-100 Preparative Ultracentrifuge analyse?

Suitable samples: Nanoparticle suspensions, Carbon nanotube and graphene dispersions, Exosomes and extracellular vesicles, Viruses and viral vectors, Liposomes and lipid nanoparticles, and Protein, nucleic acid and macromolecule solutions.

Which NAKTEM services use Beckman Coulter Optima XPN-100 Preparative Ultracentrifuge?

Ultracentrifugation and Separation.

What results do I receive?

Typical deliverables: Separated and labelled fractions (pellet, supernatant or gradient bands), Process record covering rotor, speed (rpm/g), run time, temperature and gradient composition, Volume and mass balance with recovery notes, Post-separation particle size or concentration verification on request, and Process summary report (PDF).

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